Noaansf Ecohab Heterosigma Ctd | Last Updated 5 Jun 2017

InPort Dataset ID: 17794 InPort Entity ID: 36788 Over one half of the worlds fish production for human consumption currently comes from aquaculture, while wild fisheries yields are either stable or declining. Recurring threats from the raphidophyte, Heterosigma akashiwo Hada (Sournia) have caused extensive damage ($2-6 million per episode) to wild and net-penned fish of Puget Sound, Washington, and are believed to be increasing in scope and magnitude in this region, and elsewhere in the world over the past two decades. The mechanism of H. akashiwo toxicity is not well understood. The toxic activity of H. akashiwo has been attributed to the production of reactive oxygen species, brevetoxin-like compound(s), excessive mucus, or hemolytic activity; however these mechanisms are not confirmed consistently in all fish-killing events or cultured strains. The difficulty of conducting research with active, toxin-producing field populations of H. akashiwo have resulted in conflicting findings from those obtained in lab culture studies, thereby limiting the ability of fish farmers to respond to these episodic blooms. Collaborators in this project are: Vera Trainer (NWFSC), William Cochlan (San Francisco State University), Charles Trick (University of Western Ontario), and Mark Wells (University of Maine). The overall goal of this project is to identify the primary toxic element and the specific environmental factors that stimulate fish-killing H. akashiwo blooms, and thereby provide managers with the fundamental tools needed to help reduce the frequency and toxic magnitude of these harmful algal events. Studies to date have provided incomplete and conflicting observations on the mode of toxicity and the environmental stimulation of toxification. We propose a three-pronged approach to study the environmental controls of H. akashiwo growth and toxin production; laboratory culture experiments, field observations, and bottle and mesocosm manipulation experiments.The project objectives are to: 1. identify the element(s) of toxic activity (inorganic, organic, or synergistic) associated with blooms of H. akashiwo and the various cellular morphologies of this alga, 2. determine the environmental parameters that stimulate the growth success and expression of cell toxicity in the H. akashiwo populations of Puget Sound. Because previous studies have used H. akashiwo cultures with little or no toxic activity, our approach is to use a living laboratory to study H. akashiwo bloom ecology and toxicity using natural assemblages. Using a mobile lab at field sites where H. akashiwo cells are regularly found will enable us to fully characterize the toxic element(s) responsible for fish mortality, and the environmental factors influencing toxicity. Findings from annual field studies in June and two rapid response deployments during major bloom events will be confirmed using laboratory studies with fresh ( 6 mo. old) isolates. The expected results are: 1. determination of the key elements of toxicity of H. akashiwo, 2. characterization of the environmental variables that influence either the induction or depression of elements of toxic activity in H. akashiwo, 3. characterization of environmentally-induced metabolites corresponding to condition of toxin production (metabolomics) and 4. design of a strategy for realistic mitigation of H. akashiwo activities in Puget Sound, Washington. This is a stand-alone project funded for 3 years through the NOAA/NSF ECOHAB program.

Tags: marine toxin

This dataset has the following 21 columns:

Column NameAPI Column NameData TypeDescriptionSample Values
Eventevent_nametextThe name of the individual cruise or event in which samples were collected for the overall project.
Event Start Dateevent_start_datecalendar_dateThe date on which the individual cruise or event began (local time).
Event End Dateevent_end_datecalendar_dateThe date on which the individual cruise or event ended (local time).
Stationstation_idtextThe name or identification number of a given station at which samples were collected.
Sampling Datesampling_datecalendar_dateThe date (local time) on which as sample was collected.
Sampling Start Timesampling_start_timecalendar_dateThe time (local time) on the date in which a sample began to be collected.
Sampling End Timesampling_end_timecalendar_dateThe time (local time) on the date in which a sample was finished being collected.
LatitudelatitudenumberThe latitude of the sampling station in decmal degrees.
LongitudelongitudenumberThe longitude of the sampling station in decimal degrees.
Station Depth (M)station_depthnumberThe water depth at a given station.
CastNumctd_cast_numbernumberThe number of the CTD cast performed on a specific date.
Depth(m)depth_binnumberThe depth at which the data were collected.
Pr(db)pressure_binnumberThe pressure reading at which the data were collected.
Dmdynamic_metersnumberA relative measurement of the depth at which the samples were collected using a specified reference depth.
ScansscansnumberThe number of data scans that were averaged to determine the data value for a given depth bin.
FlfluorescencenumberThe fluorescence reading from the chlorophyll fluourometer.
Calib Chl Acalibrated_chlorophyll_anumberChlorophyll a values determined using the equation of a line derived from a regression of extracted chlorphyll a analyses and fluorometer voltage.
TtemperaturenumberWater temperature.
SsalinitynumberSeawater salinity.
CondconductivitynumberSeawater conductivity.
Stsigma_tnumberSeawater density.